563 CsA-induced hypertrichosis might be caused in part by inhibition of TGF-β2 expression in dermal papilla cells.

Cyclosporin A (CsA), an immunosuppressant, is known to exhibit hypertrichosis as a side effect. This compound prolongs human hair growth in follicle organ culture system. Thus, CsA has anagen-prolonging activity, but the mechanism of action unclear. In immunosuppression, binds cyclophilin (CyP) form complex that inhibits activity phosphatase, calcineurin (CaN), which turn nuclear translocation NFAT, transcription factor involved expression IL-2 and other proteins. The purpose this study was determine how causes hypertrichosis. study, cultured dermal papilla cells (DPCs) were used. These 10% FBS-DMEM (Thermo Fisher Scientific) basic condition. Cells at 37°C humidified atmosphere containing 5% carbon dioxide gas. When assessing effect on gene expression, DPCs medium without FBS. Gene analysis carried out by PCR real-time PCR, these experiments performed with rTaq (Toyobo) or SYBR qPCR mix (Toyobo). used CaN inhibitor, VIVIT (Cayman Chemical) NFAT inhibitor. By using it evaluated seven isoforms CyP expressed DPCs. Real-time revealed CyP-A CyP-B accounted for approximately 98% CyPs. DPCs, expressions all confirmed PCR. Addition decreased TGF-β2 expression. same result obtained when added. From results, anagen decreasing Furthermore, CyPs, CaNs, NFATs, key molecules immunosuppressive mechanisms, Based intracellular signaling suppress might be similar immunosuppression.